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| 产品名称： | Psi2 13s1 |
|---|---|
| 商品货号： | TS211079 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | NIH/Swiss |
| Applications： | This construct has been used to immortalize primary cells of rat brain. The line was produced by inserting the E1A 13S cDNA sequence into the retrovirus vector DOL. The retroviruses produced during the first 24 hours post-transfection were harvested and used to infect fresh Psi2 cells to generate the Psi2 13S1 line. The retrovirus produced by this line can be used to to immortalize primary cells of rat brain. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The line was produced by inserting the E1A 13S cDNA sequence into the retrovirus vector DOL. The retroviruses produced during the first 24 hours post-transfection were harvested and used to infect fresh Psi2 cells to generate the Psi2 13S1 line. The retrovirus produced by this line can be used to to immortalize primary cells of rat brain. |
| HeLa Markers： | N |
| Genes Expressed： | a retrovirus vector containing the adenovirus 13S E1A and the neomycin phosphotransferase genes,early-region 1A, 13S E1A |
| Cellular Products： | a retrovirus vector containing the adenovirus 13S E1A and the neomycin phosphotransferase genes |
| Comments： | The 13S E1A mRNA encoded by rv13S (TS211079) is 46 amino acids longer than the 12S E1A mRNA encoded by rv12S (ATCC CRL-1808). This construct has been used to immortalize primary cells of rat brain. The line was produced by inserting the E1A 13S cDNA sequence into the retrovirus vector DOL. The resulting vector DNA (rv13S) was transfected into Psi2 cells by calcium phosphate precipitation. The retroviruses produced during the first 24 hours post-transfection were harvested and used to infect fresh Psi2 cells to generate the Psi2 13S1 line. The retrovirus produced by this line can be used to to immortalize primary cells of rat brain. Unlike Psi2 12S6 (ATCC CRL 1808), only slight induction of growth is induced in liver cultures, and no proliferative response was seen with kidney, heart, pancreas adrenal, and thyroid cultures. Tested and found negative for ectromelia virus (mousepox). The insert contains sequences from adenovirus type 2-adenovirus type 5 hybrid cDNAs. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:40 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin solution for several minutes, remove trypsin and allow the cells to sit at 37C until they begin to detach (about 3 to 5 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | RD Cone |
| References： | Cone RD, et al. A retrovirus expressing the 12S adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissue. Mol. Cell. Biol. 8: 1036-1044, 1988. PubMed: 2966895 |