微信小陈
微信小章
产品简介
购买须知
| 产品名称： | Psi2 DAP |
|---|---|
| 商品货号： | TS211081 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | embryo |
| Strain： | NIH/Swiss |
| Applications： | This line produces a vector that can infect mouse cells. Psi2 DAP cells produce a vector that encodes the human placental alkaline phosphatase gene and the neomycin resistance gene. The line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP). Cells infected with the vector produced by Psi2 DAP cells express histochemically detectable alkaline phosphatase which can be used as a marker to follow their fate in vivo. The cells may produce helper virus. Lineage analysis and immortalization of neural cells via retrovirus vectors in Neuromethods, Molecular Neurobiological Techniques 16:117-219, Boulton, A.A., Baker, G.B. and Campagnoni, A.T., Eds. |
| Derivation： | The line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP). Cells infected with the vector produced by Psi2 DAP cells express histochemically detectable alkaline phosphatase which can be used as a marker to follow their fate in vivo. |
| Genes Expressed： | a human placental alkaline phosphatase transducing vector |
| Cellular Products： | a human placental alkaline phosphatase transducing vector |
| Comments： | This line produces a vector that can infect mouse cells. Psi2 DAP cells produce a vector that encodes the human placental alkaline phosphatase gene and the neomycin resistance gene. The line was derived from Psi2 cells by insertion of a recombinant retrovirus genome (DAP). Cells infected with the vector produced by Psi2 DAP cells express histochemically detectable alkaline phosphatase which can be used as a marker to follow their fate in vivo. The cells may produce helper virus. To assay for helper virus, and for other retrovirus techniques, see Cepko, C.L. Lineage analysis and immortalization of neural cells via retrovirus vectors in Neuromethods, Molecular Neurobiological Techniques 16:117-219, Boulton, A.A., Baker, G.B. and Campagnoni, A.T., Eds., Humana Press, Clifton, NJ, 1989. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:50 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor： | CL Cepko |
| Deposited As： | Mus musculus |
| References： | Fields-Berry SC, et al. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc. Natl. Acad. Sci. USA 89: 693-697, 1992. PubMed: 1731342 |