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微信小章| 产品名称： | Pt K1 (NBL-3) PTK1 |
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| 商品货号： | TS211082 |
| Organism： | Potorous tridactylus, potoroo |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | keratin |
| Cellular Products： | keratin |
| Virus Resistance： | poliovirus 2 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 4th edition, published by Wiley -Liss, N.Y., 2000. |
| Name of Depositor： | KH Walen |
| Deposited As： | Potorous tridactylis |
| References： | . . Nature 194: 406, 1962. . . Cytogenetics 3: 19, 1964. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |