微信小陈
微信小章| 产品名称： | PT67 10A1 MLV-based retrovirus packaging cell line |
|---|---|
| 商品货号： | TS211084 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 2 The DNA construct used to make the cells does contain a polyadenylation signal from SV-40, but this has no oncogenic or other functional consequence. None of the transforming genes of SV-40 are present. PT67 cells have been used to make retroviral vectors for human gene therapy purposes, and need not be handled at Biosafety Level 2.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Can be used to generate retroviral vectors with a broad host range, including humans. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | PT67 is a retrovirus packaging cell line derived from TK- NIH/3T3 cells. |
| Comments： | PT67 is a retrovirus packaging cell line derived from TK- NIH/3T3 cells. The cells produce replication-defective retroviral vector particles capable of binding to the gibbon ape leukemia virus receptor Glvr-1 (Pit-1) or the amphotropic retrovirus receptor Ram-1 (Pit-2) on target cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:12 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | Fred Hutchinson Cancer Res. Cntr. |
| Deposited As： | mouse |
| U.S. Patent Number： | |
| References： | Miller AD, Chen F. Retrovirus packaging cells based on 10AI murine leukemia virus for production of vectors that use multiple receptors for cell entry. J. Virol. 70: 5564-5571, 1996. PubMed: 8764070 Miller AD. 10A1 Retroviral packaging cells and uses thereof. US Patent 5,766,945 dated Jun 16 1998 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |