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| 产品名称： | P-tyr-1 |
|---|---|
| 商品货号： | TS211086 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with phosphotyrosine and proteins containing phosphotyrosine. The antibody does not react with phosphothreonine or phosphoserine, and is useful in immunoprecipitation and western blot assays. |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against phosphotyrosine |
| Cellular Products： | immunoglobulin; monoclonal antibody; against phosphotyrosine |
| Comments： | Animals were immunized with aminobenzylphosphonate conjugated to hemocyanin. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with phosphotyrosine and proteins containing phosphotyrosine. The epitope recognized by P-tyr-1 appears to be on the phenyl phosphate group of phosphotyrosine. The antibody does not react with phosphothreonine or phosphoserine, and is useful in immunoprecipitation and western blot assays. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | JK Klarlund |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Klarlund JK. Transformation of cells by an inhibitor of phosphatases acting on phosphotyrosine in proteins. Cell 41: 707-717, 1985. PubMed: 2408760 Klarlund JK, et al. Numerous proteins phosphorylated on tyrosine and enhanced tyrosine kinase activities in vanadate-treated NIH 3T3 fibroblasts. Biochim. Biophys. Acta 971: 112-120, 1988. PubMed: 3408742 |