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微信小章| 产品名称： | PU5-1.8 (PU5-1R) |
|---|---|
| 商品货号： | TS211087 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Culture Properties： | suspension (some adherent cells) |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoid tumor |
| Strain： | BALB/c |
| Applications： | The line is sensitive to growth inhibition by LPS and PPD. Tested and found negative for ectromelia virus (mousepox). |
| Receptor Expression： | complement (C3) |
| Genes Expressed： | lysozyme; granulocyte colony stimulating activity (CSA) inducible by LPS |
| Cellular Products： | lysozyme; granulocyte colony stimulating activity (CSA) inducible by LPS |
| Comments： | PU5-1.8 cells phagocytose latex beads and zymosan. The cells are capable of antibody dependent lysis of both sheep erythrocytes and tumor cells. The line is sensitive to growth inhibition by LPS and PPD. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; horse serum, 10%
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Adherent cells can be recovered by scraping. |
| Name of Depositor： | P Ralph |
| Deposited As： | Mus musculus |
| References： | Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890 Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031 Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922 Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed: 4208429 |