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| 产品名称： | PS 38 |
|---|---|
| 商品货号： | TS211092 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody cross-reacts with the NP-OT of both mouse in both radioimmunoassays and immunohistochemical assays. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against rat oxytocin associated neurophysin (NP-OT) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against rat oxytocin associated neurophysin (NP-OT) |
| Comments： | Animals were immunized with an acid soluble extract of rat (Sprague-Dawley) intermediate lobes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody cross-reacts with the NP-OT of both mouse in both radioimmunoassays and immunohistochemical assays. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, non-essential amino acids, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; heat-inactivated fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2b; kappa light chain |
| Name of Depositor： | H Gainer |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ben-Barak Y, et al. Neurophysin in the hypothalamo-neurohypophysial system. I. Production and characterization of monoclonal antibodies. J. Neurosci. 5: 81-97, 1985. PubMed: 3880813 Ben-Barak Y, et al. Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin. Cell. Mol. Neurobiol. 4: 339-349, 1984. PubMed: 6085284 |