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微信小章| 产品名称： | PMJ2-PC |
|---|---|
| 商品货号： | TS211098 |
| Organism： | Mus musculus, mouse |
| Cell Type： | peritoneal macrophage; infected with J2 viru |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | suspension; some adherent cells |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Strain： | C57BL/6J |
| Applications： | Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines. Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells. The cells are phagocytic, non-specific esterase positive and are Fc receptor positive. The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Antigen Expression： | MHC class II +; MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 - ; Lyt-1.2 -; Thy-1.2 -; sIg - |
| Genes Expressed： | MHC class II +; MAC-1 (CD11b) +; MAC-2 +; Fc receptor (FcR) +; F4/80 +; Ly-5 +; Lyt-1.1 - ; Lyt-1.2 -; Thy-1.2 -; sIg - |
| Comments： | PMJ2-PC (TS211098) and AMJ2-R (ATCC CRL-2458) are cloned, continuous, peritoneal macrophage cell lines generated from C57BL6J mice by in vivo infection with the J2 retrovirus carrying the v-raf and v-myc oncogenes. Cell staining demonstrated intracellular expression of the product of the v-raf gene in these cell lines. The in vivo immortalized cells had many of the morphological and functional characteristics of peritoneal macrophages. Analysis demonstrated the presence of peritoneal macrophage associated cell surface antigens, interleukin-6 (IL-6) secretion induced by lipopolysaccharide (LPS), and biological response modifier-induced cytotoxic activity against tumor cells. The cells are phagocytic, non-specific esterase positive and are Fc receptor positive. One of the clones, PMJ2-PC, constitutively expressed major histocompatibility complex (MHC) class II antigens, and in the other clone, PMJ2-R, MHC class II antigens expression was induced by recombinant murine interferon-gamma. The cells produce nitric oxide (NO) when stimulated with a mixture of rMuIFN-gamma and LPS. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 5 mM HEPES, 95%; fetal bovine serum, 5%
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| Subculturing： | Scrape off the attached cells and transfer along with the floating cells into new flasks. Medium Renewal: Twice per week |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | AV Palleroni |
| Deposited As： | mouse |
| References： | Adami C, et al. In vivo immortalization of murine peritoneal macrophages: a new rapid and efficient method for obtaining macrophage cell lines. J. Leukocyte Biol. 53: 475-478, 1993. PubMed: 7683328 Palleroni AV, et al. Nitric oxide synthase induction in lines of macrophages from different anatomical sites. Cell. Mol. Biol. 44: 527-535, 1998. PubMed: 9620450 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |