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微信小章| 产品名称： | PK136 |
|---|---|
| 商品货号： | TS211099 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | Hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | Lymphoblast-like |
| Culture Properties： | Suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | (C3H X BALB/c)F1 (B cell); BALB/c (myeloma) |
| Applications： | The antibody is specific for NK cells and is cytotoxic in the presence of complement. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with spleen and bone marrow cells from CE mice.xa0 Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse natural killer (NK) cells |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse natural killer (NK) cells |
| Comments： |
The antibody is specific for NK cells and is cytotoxic in the presence of complement.
The pattern of reactivity of the antibody with cells of various strains of mice matches the pattern of conventional NK 1.1 antisera. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: |
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cultures at a cell concentration between 1 x 105xa0and 1 x 106xa0cells/mL. Do not allow the cell concentration to exceed 1 x 106xa0cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Isotype： | IgG2a |
| Name of Depositor： | GC Koo |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Koo GC, et al. The NK-1.1 (-) mouse: a model to study differentiation of murine NK cells. J. Immunol. 137: 3742-3747, 1986. PubMed: 3782794 Koo GC, Peppard JR. Establishment of monoclonal anti-NK-1.1 antibody. Hybridoma 3: 301-303, 1984. PubMed: 6500587 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |