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微信小章| 产品名称： | PK(15) PK15, PK-15 |
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| 商品货号： | TS211100 |
| Organism： | Sus scrofa, pig |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Cellular Products： | plasminogen activator; keratin |
| Virus Susceptibility： | Classical swine fever virus
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Classical swine fever virus African swine fever virus Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana) Vaccinia virus Human adenovirus 4 Human adenovirus 5 Human Coxsackievirus B 2 Human Coxsackievirus B3 Human Coxsackievirus B4 Human Coxsackievirus B 5 Human Coxsackievirus B 6 |
| Virus Resistance： | poliovirus 2 |
| Comments： | The presence of a porcine papovavirus in PK(15) cells has been reported in cells obtained from multiple sources including the ATCC.
The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that TS211100 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. The cell line harbors an endogenous C-type retrovirus. The cells are positive for porcine circovirus (PCV) antigens. The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
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| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | Cutter Laboratories, Inc. |
| Deposited As： | Sus scrofa |
| References： | Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line (TS211100) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830 Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860 Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256 Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097 Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060 Todaro GJ, et al. Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74, 1974. PubMed: 4132403 James A House, personal communication |
| Cross References： | Nucleotide (GenBank) : AJ133817 Porcine endogenous retrovirus type C proviral gag, pol and env genesand LTR (class A, clone 42). Nucleotide (GenBank) : AJ133816 Porcine endogenous retrovirus type C proviral gag, pol and env genes and LTR (class B, clone 33). |