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| 产品名称： | PL45 |
|---|---|
| 商品货号： | TS211103 |
| Organism： | Homo sapiens, human |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | adherent with some rounded floating cells |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | ductal adenocarcinoma |
| Age： | adult |
| Gender： | male |
| Ethnicity： | White |
| Applications： | Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. PL45 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the pancreas of a man with poorly differentiated pancreatic adenocarcinoma of ductal origin. The line expresses wild-type DPC4 and BRCA2 genes. The PL45 cell line was derived from the same patient as the Panc 10.05 cell line (ATCC CRL-2547). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | PL45 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the pancreas of a man with poorly differentiated pancreatic adenocarcinoma of ductal origin. The PL45 cell line was derived from the same patient as the Panc 10.05 cell line (ATCC CRL-2547). |
| Clinical Data： | White adult male PL45 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the pancreas of a man with poorly differentiated pancreatic adenocarcinoma of ductal origin. The PL45 cell line was derived from the same patient as the Panc 10.05 cell line (ATCC CRL-2547). |
| Oncogene： | BRCA2 +; DPC4 +; K-ras +; p53 + |
| Genes Expressed： | BRCA2 +; DPC4 +; K-ras +; p53 + |
| Comments： | The MTS1 gene on chromosome 9p21 encodes the p16 inhibitor of cyclinD/CDK4 complexes. PL45 cells have a wild-type p16 gene. This gene is transcriptionally silenced and methylated in PL45 cells. PL45 cells have a wild-type cyclin-dependent kinase (CDK4) gene. Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. The line expresses wild-type DPC4 and BRCA2 genes. The line has a p53 gene mutation at codon 255 where an ATC --> AAC mutation resulted in substitution of asparagine for isoleucine. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 9,12 D5S818: 13 D7S820: 8,9 THO1: 6,9.3 TPOX: 11 vWA: 16 |
| Name of Depositor： | SE Kern |
| Deposited As： | human |
| Year of Origin： | 1992 |
| References： | Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602 Caldas C, et al. Frequent somatic mutations and homozygous deletions of the p16 (MTS1) gene in pancreatic adenocarcinoma. Nat. Genet. 8: 27-32, 1994. PubMed: 7726912 Su GH, et al. ACVR1B (ALK4, activin receptor type 1B) gene mutations in pancreatic carcinoma. Proc. Natl. Acad. Sci. USA 98: 3254-3257, 2001. PubMed: 11248065 |