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微信小章| 产品名称： | PLC/PRF/5 |
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| 商品货号： | TS211105 |
| Organism： | Homo sapiens, human |
| Tissue： | liver |
| Cell Type： | Alexander cells |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain hepatitis B
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hepatoma |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Genes Expressed： | hepatitis virus B surface antigen (HBsAg) |
| Cellular Products： | hepatitis virus B surface antigen (HBsAg) |
| Comments： | The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline. The cells secrete HBsAg. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 11,12 D16S539: 13 D5S818: 12 D7S820: 9,11 THO1: 8 TPOX: 8 vWA: 15,16 |
| Name of Depositor： | WJ McAleer |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： | |
| References： | Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321 MacNab GM, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208 Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138 Knowles B. et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209(4455): 497-499, 1980. PubMed: 6248960 Alexander J, et al. Adaptation of cells derived from human malignant tumours to growth in vitro. S. Afr. J. Med. Sci. 41(2):89-98, 1976. PubMed:184551 Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281 Alexander JJ, et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998 |
| Cross References： | Nucleotide (GenBank) : X75311 H.sapiens /Hepatitis B virus fusion mRNA for mevalonate kinase. Nucleotide (GenBank) : X77700 H.sapiens / Hepatitis B virus (A4.0) DNA for mevalonate kinase (partial). |