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微信小章| 产品名称： | pgsC-605 |
|---|---|
| 商品货号： | TS211115 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | sulfate transporter deficient |
| Gender： | female |
| Applications： | PgsC-605 is a Chinese hamster ovary cell mutant deficient in sulfate transporter. The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and isolated by replica plating 35Ssulfate colony autoradiography. PgsC-605 cells continue to produce heparin sulfate and chondroitin sulfate chains, but depend on endogenous formation of sulfate from sulfur containing amino acids. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and isolated by replica plating 35Ssulfate colony autoradiography. |
| Clinical Data： | female |
| Comments： | PgsC-605 is a Chinese hamster ovary cell mutant deficient in sulfate transporter. The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and isolated by replica plating 35Ssulfate colony autoradiography. PgsC-605 cells continue to produce heparin sulfate and chondroitin sulfate chains, but depend on endogenous formation of sulfate from sulfur containing amino acids. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | JD Esko |
| Deposited As： | Cricetulus griseus |
| References： | Esko JD, et al. Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096, 1988. PubMed: 3137658 Esko JD, et al. Sulfate transport-deficient mutants of Chinese hamster ovary cells. Sulfation of glycosaminoglycans dependent on cysteine. J. Biol. Chem. 261: 15725-15733, 1986. PubMed: 3782085 Elgavish A, et al. Chinese hamster ovary cell mutants deficient in an anion exchanger functionally similar to the erythroid band 3. J. Biol. Chem. 263: 18607-18613, 1988. PubMed: 3198592 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |