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| 产品名称： | PI 153/3 |
|---|---|
| 商品货号： | TS211117 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Neuroblastoma,Leukemia |
| Applications： | The antigen recognized by this antibody is present on fetal brain, B cell and Null cell leukemias but not on adult brain. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a glycoprotein expressed on human neuroectoderm derived tumors (neuroblastoma, retinoblastoma and glioblastoma) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a glycoprotein expressed on human neuroectoderm derived tumors (neuroblastoma, retinoblastoma and glioblastoma) |
| Comments： | Animals were immunized with the human neuroblastoma line IMR6. Spleen cells were fused with P3X63Ag8 myeloma cells. The antigen recognized by this antibody is present on fetal brain, B cell and Null cell leukemias but not on adult brain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | R Kennett |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Kennett RH, Gilbert F. Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain. Science 203: 1120-1121, 1979. PubMed: 424740 |