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| 产品名称： | PDL-1 |
|---|---|
| 商品货号： | TS211122 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the cell surface of periodontal ligament cells of bovine and baboon origin. The antibody also reacts with cells of mesenchymal origin from a number of organs. |
| Derivation： | Spleen cells were fused with FOX-NY myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a cell surface antigen on bovine periodontal ligament cells |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a cell surface antigen on bovine periodontal ligament cells |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with cultured bovine periodontal ligament cells. Spleen cells were fused with FOX-NY myeloma cells. The antibody reacts with the cell surface of periodontal ligament cells of bovine and baboon origin. The antibody also reacts with cells of mesenchymal origin from a number of organs. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | RJ Klebe |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | DuBois WT, et al. Monoclonal antibodies to periodontal ligament cells. J. Periodontol. 62: 190-196, 1991. PubMed: 2027070 |