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微信小章| 产品名称： | PEA 10 |
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| 商品货号： | TS211123 |
| Organism： | Mus musculus, mouse |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | LRP deficient |
| Age： | embryo; 15 days gestation |
| Applications： | This cell line is a suitable transfection host. This line along with the MEF-1 (see ATCC CRL-2214) and PEA 13 (see ATCC CRL-2216) cell lines constitute a genetically defined system to study the endocytosis of ligands by the LRP. These cell lines are the only available experimental system to study the effect of LRP deficiency because LRP deficient mouse embryos die in utero. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The cell line was initiated in 1993 by selection with Pseudomonas exotoxin A (PEA). |
| Comments： | PEA 10 cells are heterozygous for a disruption of the gene for low density lipoprotein receptor related protein (LRP).
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | TE Willnow |
| Deposited As： | Mus musculus |
| Year of Origin： | 1993 |
| References： | Kounnas MZ, et al. LDL receptor-related protein, a multifunctional ApoE receptor, binds secreted beta-amyloid precursor protein and mediates its degradation. Cell 82: 331-340, 1995. PubMed: 7543026 Willnow TE, Herz J. Genetic deficiency in low density lipoprotein receptor-related protein confers cellular resistance to Pseudomonas exotoxin A. Evidence that this protein is required for uptake and degradation of multiple ligands. J. Cell Sci. 107: 719-726, 1994. PubMed: 8006085 Orth K, et al. Low density lipoprotein receptor-related protein is necessary for the internalization of both tissue-type plasminogen activator-inhibitor complexes and free tissue-type plasminogen activator. J. Biol. Chem. 269: 21117-21122, 1994. PubMed: 8063731 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |