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购买须知
| 产品名称： | Pfeiffer |
|---|---|
| 商品货号： | TS211125 |
| Organism： | Homo sapiens, human |
| Tissue： | derived from metastatic site: pleural effusion |
| Cell Type： | lymphoblast B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | diffuse large cell lymphoma; non-Hodgkins B cell lymphoma |
| Age： | adult |
| Gender： | male |
| Ethnicity： | White |
| Applications： | This cell line is a model system for studying non-Hodgkin lymphomas. |
| Karyotype： | The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas. |
| Derivation： | The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei.
|
| Clinical Data： | male
White
adult |
| Antigen Expression： | CD10; Homo sapiens CD19; Homo sapiens CD20; Homo sapiens CD38; Homo sapiens CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - Ref   Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Comments： | The cells are negative for Epstein-Barr virus. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 - 5 x 105 viable cells/mL. Maintain cell density between 3 x 105 and 3 x 106 viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 12 D5S818: 10, 13 D7S820: 8, 12 THO1: 9, 9.3 TPOX: 9 vWA: 17, 18 Amelogenin: XY |
| Isotype： | IgG kappa |
| Population Doubling Time： | 24 to 30 hrs |
| Name of Depositor： | H Ben-Bassat |
| Deposited As： | human |
| Year of Origin： | 1992 |
| References： | Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |