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微信小章| 产品名称： | PFSK-1 |
|---|---|
| 商品货号： | TS211126 |
| Organism： | Homo sapiens, human |
| Tissue： | brain, cerebral hemisphere |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | malignant primitive neuroectodermal tumor |
| Age： | 22 months |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | hypotetraploid; 84, XXY; -Y, t(Xp;8q), del(1)(p22), -3, del(4)(p14), -9, -10, -13, -14, -14, -16, -22 |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c nu/nu mice |
| Comments： | PFSK-1 cells form colonies in soft agar, and lack contact inhibition. They express the intermediate filament protein, nestin, and are positive for neuron specific enolase (NSE). They lack characterisics of terminally differentiated neurons or glia. Restriction fragment length polymorphism studies showed loss of heterozygosity for multiple loci on chromosome 17. Neither c-myc nor N-myc is amplified or re-arranged. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11 D13S317: 12,14 D16S539: 13 D5S818: 11,12 D7S820: 10 THO1: 9,9.3 TPOX: 8,9 vWA: 17,18 |
| Population Doubling Time： | 30 hrs |
| Name of Depositor： | D Fults |
| Deposited As： | Homo sapiens |
| References： | Fults D, et al. Establishment and characterization of a human primitive neuroectodermal tumor cell line from the cerebral hemisphere. J. Neuropathol. Exp. Neurol. 51: 272-280, 1992. PubMed: 1316433 Cote GJ, et al. Sequence requirements for regulated RNA splicing of the human fibroblast growth factor receptor-1 alpha exon. J. Biol. Chem. 272: 1054-1060, 1997. PubMed: 8995402 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |