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微信小章| 产品名称： | PFHR 9 PFHR-9 |
|---|---|
| 商品货号： | TS211127 |
| Organism： | Mus musculus, mouse |
| Cell Type： | Epithelial,Epithelial-like |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | embryonal carcinoma |
| Age： | embryo |
| Applications： | PFHR 9 (PFHR-9)is a differentiated cell line isolated as a HAT-resistant colony of the undifferentiated mouse embryonal carcinoma PCC4F in 1974. The PFHR 9 cells were not tumorigenic when injected subcutaneously into 129/Sv mice whereas the parental line did produce tumors. The cells produce basement membrane proteins - laminin-1; entactin-1; perlecan and collagen IV. The cell line is useful for the production of basement membrane proteins and as a model to study the assembly and secretion of these proteins. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | PFHR 9 (PFHR-9)is a differentiated cell line isolated as a HAT-resistant colony of the undifferentiated mouse embryonal carcinoma PCC4F in 1974. |
| Genes Expressed： | laminin-1; entactin-1; perlecan and collagen IV |
| Cellular Products： | laminin-1; entactin-1; perlecan and collagen IV |
| Tumorigenic： | No |
| Effects： | No, not tumorigenic when injected subcutaneously into 129/Sv mice |
| Comments： | PFHR 9 (PFHR-9)is a differentiated cell line isolated as a HAT-resistant colony of the undifferentiated mouse embryonal carcinoma PCC4F in 1974. While PCC4F cells grow in suspension, the PRHR cell line is adherent. The cells are epithelial-like and have numerous vacuoles. PFHR 9 cells have a reported doubling time of 12 hours. The PFHR 9 cells were not tumorigenic when injected subcutaneously into 129/Sv mice whereas the parental line did produce tumors. The cells produce basement membrane proteins - laminin-1; entactin-1; perlecan and collagen IV. The cell line is useful for the production of basement membrane proteins and as a model to study the assembly and secretion of these proteins. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:7 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 12 hrs |
| Name of Depositor： | A Chung |
| Deposited As： | mouse |
| References： | Chung AE, et al. Morphological and biochemical observations on cells derived from the in vitro differentiation of the embryonal carcinoma cell line PCC4-F. Cancer Res. 37: 2072-2081, 1977. PubMed: 558822 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |