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微信小章| 产品名称： | PC-12 |
|---|---|
| 商品货号： | TS211132 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | adrenal gland |
| Product Format： | frozen |
| Morphology： | small irregularly shaped cells |
| Culture Properties： | floating clusters; few scattered lightly attached cells. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pheochromocytoma |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | 40 chromosomes; 38 autosomes plus XY
Ref  Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 |
| Images： |  |
| Derivation： | The PC-12 cell line was derived from a transplantable rat pheochromocytoma. |
| Clinical Data： | male |
| Receptor Expression： | nerve growth factor (NGF), expressed |
| Genes Expressed： | catecholamines; dopamine; norepinephrine |
| Cellular Products： | catecholamines; dopamine; norepinephrine |
| Tumorigenic： | Yes |
| Effects： | Yes, in New England Deaconess Hospital strain rats |
| Comments： | The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks. The cells do not synthesize epinephrine. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Protocol: Volumes used for this protocol are for a 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time： | 48 hrs |
| Name of Depositor： | B Patterson |
| Deposited As： | Rattus sp. |
| References： | Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317 Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712 Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125 U.S. Pharmacopeia USP Monographs: Small Intestinal Submucosa Wound Matrix. Rockville, MD. USP32-NF27, 2005 Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897 |
| Cross References： | Nucleotide (GenBank) : AF311055 Rattus norvegicus thioredoxin mRNA, complete cds. Nucleotide (GenBank) : AF319950 Rattus norvegicus glutaredoxin mRNA, complete cds. Nucleotide (GenBank) : AF311054 Rattus norvegicus APEX (Apex) mRNA, partial cds, alternatively spliced. |