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微信小章| 产品名称： | PC 61 5.3 PC 61, PC 61.5.3 |
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| 商品货号： | TS211133 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue： | Spleen |
| Cell Type： | Hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | Lymphoblast-like |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | OFA (B cell); BALB/c (myeloma) |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with the B6.1 mouse cytotoxic T cell line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse interleukin-2 (interleukin 2, IL-2) receptor (CD25) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse interleukin-2 (interleukin 2, IL-2) receptor (CD25) |
| Comments： | The antibody blocks IL-2 binding and IL-2 dependent growth stimulation, and immunoprecipitates IL-2 receptors. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/mL and maintain between 1 x 105 and 5 x 105 cells/mL.
Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density). |
| Cryopreservation： | Complete growth medium 92.5%; DMSO, 7.5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 , IgG1 |
| Name of Depositor： | M Nabholz |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Trowbridge IS, et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J. Cell. Physiol. 112: 403-410, 1982. PubMed: 6290505 Zubler RH, et al. Activated B cells express receptors for, and proliferate in response to, pure interleukin-2. J. Exp. Med. 160: 1170-1183, 1984. PubMed: 6434689 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |