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微信小章| 产品名称： | PANC-1 |
|---|---|
| 商品货号： | TS211134 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas/duct |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | epithelioid carcinoma |
| Age： | 56 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Chromosome studies indicate a modal number of 63 with 3 distinct marker chromosomes and a small ring chromosome. This is a hypertriploid human cell line. The modal chromosome number was 61, occurring in 32% of cells., However, cells with 63 chromosomes also occurred at a high frequency (22%). The rate of cells with higher ploidies was 8.5%. |
| Images： |  |
| Clinical Data： | 56 years Caucasian male |
| Comments： | Growth is inhibited by 1 unit/mL L-asparaginase.
The cells will grow in soft agar. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Subcultivation Ratio:xa0A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal:xa02 to 3 times per week |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 11,13 D13S317: 11 D7S820: 8,10 D16S539: 11 vWA: 15 THO1: 7,8 Amelogenin: X TPOX: 8,11 CSF1PO: 10,12 |
| Isoenzymes： | G6PD, B |
| Population Doubling Time： | 52 hrs |
| Name of Depositor： | M Lieber |
| Deposited As： | Homo sapiens |
| References： | Lieber M, et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870 Wu MC, et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626 Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 |