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微信小章| 产品名称： | PA317 LXSN 6E6 |
|---|---|
| 商品货号： | TS211142 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain human papilloma viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Papilloma |
| Age： | embryo |
| Strain： | NIH/Swiss |
| Applications： | Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418. The pLXSN6bE6 vector contains the human papilloma virus (HPV) type 6b E6 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter - enhancer sequences. The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter. This line produces the amphotropic retrovirus LXSN6bE6 which encodes the HPV6b E6 open reading frame, and which can be used to stably infect many cell types. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | PA317 LXSN 6E6 is a packaging cell line developed by transfection of the retrovirus vector pLXSN6bE6 into the Psi-2 ecotropic packaging cell line. Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418. |
| Comments： | PA317 LXSN 6E6 is a packaging cell line developed by transfection of the retrovirus vector pLXSN6bE6 into the Psi-2 ecotropic packaging cell line. Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418. The pLXSN6bE6 vector contains the human papilloma virus (HPV) type 6b E6 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter - enhancer sequences. The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter. This line produces the amphotropic retrovirus LXSN6bE6 which encodes the HPV6b E6 open reading frame, and which can be used to stably infect many cell types. The resulting cells constitutively express the E6 protein of HPV6b. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:6 to 1:12 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | DA Galloway |
| Deposited As： | Mus musculus |
| References： | Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |