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| 产品名称： | PAb 100 (formerly clone 7) |
|---|---|
| 商品货号： | TS211145 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with lower affinity than PAb 101 (ATCC TIB-117) and precipitates large T antigen and NVT antigen (when complexed) but not small t antigen or the SV-40 small t antigen. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against large T antigen (SV40) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against large T antigen (SV40) |
| Comments： | Animals were immunized with SVT-40, an SV40 transformed BALB/c cell line. Spleen cells were fused with NS-1 mouse myeloma cells. The antibody recognizes a denaturation sensitive determinant unique to large T (tumor) antigen. The antibody reacts with lower affinity than PAb 101 (ATCC TIB-117) and precipitates large T antigen and NVT antigen (when complexed) but not small t antigen or the SV-40 small t antigen. The antibody does not bind to denatured large T or mouse NVT in radioimmunoassays. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. |
| Culture Conditions： | Temperature: 37°C |
| Isotype： | IgG1 |
| Name of Depositor： | EG Gurney |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Deppert W, et al. Monoclonal antibodies against simian virus 40 tumor antigens: analysis of antigenic binding sites, using adenovirus type 2-simian virus 40 hybrid viruses. J. Virol. 37: 478-482, 1981. PubMed: 6163870 Gurney EG, et al. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct subclasses of large T antigen and for similarities among nonviral T antigens. J. Virol. 34: 752-763, 1980. PubMed: 6155477 |