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| 产品名称： | PAb 108 |
|---|---|
| 商品货号： | TS211146 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody cross-reacts with the large T antigen of BK virus. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a determinant on the N terminal region of large T antigen and small t antigen (SV40) |
| Comments： | Animals were immunized with SV40 transformed BALB/3T3 cells. Spleen cells were fused with NS-1 myeloma cells. The antibody cross-reacts with the large T antigen of BK virus. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 80%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | EG Gurney |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Gurney EG, et al. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J. Virol. 57: 1168-1172, 1986. PubMed: 2419584 |