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| 产品名称： | p53NiS1 |
|---|---|
| 商品货号： | TS211148 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | malignant fibrous histiocytoma |
| Age： | adult |
| Applications： | The p53NiS1 cell line was derived in June 1997 by explant culture of a primary malignant fibrous histiocytoma.
The cell line demonstrates hypermethylation of the tumor suppressor p16, loss of heterozygosity of p53, and activation of the MAP kinase signaling pathway. |
| Derivation： | The p53NiS1 cell line was derived in June 1997 by explant culture of a primary malignant fibrous histiocytoma. The tumor developed three months after implantation of nickel sulfide (NiS) intramuscularly into the right rear leg of a p53 heterozygous mouse. |
| Oncogene： | p53 (deletion in exon 5); p16 -; p19ARF + |
| Genes Expressed： | p53 (deletion in exon 5); p16 -; p19ARF + |
| Tumorigenic： | No |
| Effects： | No, tumors did not develop within four months in nude mice (3/3) inoculated with 1 X 10 exp6 cells |
| Comments： | The p53NiS1 cell line was derived in June 1997 by explant culture of a primary malignant fibrous histiocytoma. The tumor developed three months after implantation of nickel sulfide (NiS) intramuscularly into the right rear leg of a p53 heterozygous mouse.
The cell line demonstrates hypermethylation of the tumor suppressor p16, loss of heterozygosity of p53, and activation of the MAP kinase signaling pathway. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:5 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JL Arbiser |
| Deposited As： | mouse |
| Year of Origin： | 1997 |