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| 产品名称： | P3X63Ag8U.1 |
|---|---|
| 商品货号： | TS211154 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Gender： | female |
| Strain： | BALB/c |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was derived from the MOPC 21 - P3X63Ag8 lineage. |
| Clinical Data： | female |
| Antigen Expression： | H-2d |
| Comments： | The cells do not synthesize gamma 1 chains.
They produce but do not secrete kappa chains.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4xa0x 105 viable cells/mL and maintain below 1xa0x 106 cells/mL.
Medium Renewal: 3 times per week |
| Cryopreservation： | Freeze medium: Culture medium, 95%; DMSO, 5%. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | MD Scharff |
| Deposited As： | Mus musculus |
| References： | Yelton DE, et al. Fusion of mouse myeloma and spleen cells. Curr. Top. Microbiol. Immunol. 81: 1-7, 1978. PubMed: 567551 |
| Cross References： | Nucleotide (GenBank) : E02974 DNA encoding mouse interleukin-6(IL-6) receptor. |