微信小陈
微信小章| 产品名称： | P388D1 P388 D1 |
|---|---|
| 商品货号： | TS211158 |
| Organism： | Mus musculus, mouse |
| Cell Type： | monocyte; macrophage |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | DBA/2 |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.xa0 |
| Comments： | A subclone of this line see ATCC TIB-63, P388D1(IL-1) produces high levels of interleukin-1 (IL-1, interleukin 1). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 5 x 105 viable cells/mL.
A maximum of 6 x 106 viable cells/mL is obtainable. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C |
| Name of Depositor： | EM Jensen |
| Deposited As： | Mus musculus |
| References： | Dawe CJ, Potter M. Morphologic and biologic progression of a lymphoid neoplasm of the mouse in vivo and in vitro. Am. J. Pathol. 33: 603, 1957 Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03. |
| Cross References： | Nucleotide (GenBank) : U88624 Mus musculus 85 kDa calcium-independent phospholipase A2 mRNA, complete cds. |