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微信小章| 产品名称： | P3.6.2.8.1 |
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| 商品货号： | TS211162 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma). Tested and found negative for ectromelia virus (mousepox). |
| Karyotype： | near tetraploid |
| Derivation： | This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma). |
| Antigen Expression： | H-2d; PC.1 |
| Genes Expressed： | immunoglobulin; monoclonal antibody,H-2d; PC.1 |
| Cellular Products： | immunoglobulin; monoclonal antibody |
| Comments： | This line is a clonal derivative of the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma). The cells bear surface immunoglobulin (sIg+). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | P Knopf, A Destree, R Hyman |
| Deposited As： | Mus musculus |
| References： | Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Knopf PM, et al. Antibody-induced changes in expression of an immunoglobulin surface antigen. Eur. J. Immunol. 3: 351-359, 1973. PubMed: 4758896 Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Kimmel CB. Immunoglobun, protein and nucleic acid synthesis in cultured myeloma cells. Exp. Cell Res. 65: 202-208, 1971. PubMed: 4101162 Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516 Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914 |