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微信小章| 产品名称： | P388D1 (IL-1) |
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| 商品货号： | TS211163 |
| Organism： | Mus musculus, mouse |
| Cell Type： | methylcholanthrene induced |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoid tumor |
| Strain： | DBA/2 |
| Applications： | The cells are negative for surface immunoglobulin (sIg-). Tested and found negative for ectromelia virus (mousepox). |
| Receptor Expression： | complement (C3) |
| Genes Expressed： | interleukin-1 (interleukin 1, IL-1 or LAF) induced by LPS or phorbol myristic acid (PMA, TPA); lysozyme |
| Cellular Products： | interleukin-1 (interleukin 1, IL-1 or LAF) induced by LPS or phorbol myristic acid (PMA, TPA); lysozyme |
| Comments： | These cells phagocytose zymosan and latex beads. They are active in antibody dependent cell mediated cytotoxicity systems. The cells are negative for surface immunoglobulin (sIg-). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 3 times per week Subcultures are prepared by scraping. Remove old medium, add fresh dislodge the cells and dispense into new flasks. |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | S Mizel |
| Deposited As： | Mus musculus |
| References： | Mizel SB. Physicochemical characterization of lymphocyte-activating factor (LAF). J. Immunol. 122: 2167-2172, 1979. PubMed: 312860 Mizel SB. Biochemical and biological characterization of lymphocyte-activating factor (LAF) produced by the murine macrophage cell line, P388D1. Ann. N.Y. Acad. Sci. 332: 539-549, 1979. PubMed: 231409 Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031 Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922 Koren HS, et al. Identification of macrophage-like characteristics in a cultured murine tumor line. J. Immunol. 114: 894-897, 1975. PubMed: 1167564 . Manual of macrophage methodology. New York: Marcel Dekker; 1981. |