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微信小章| 产品名称： | P19 |
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| 商品货号： | TS211164 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | teratocarcinoma; embryonal carcinoma |
| Gender： | male |
| Strain： | C3H/He |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | n = 40; XY, n = 40; XY
Ref  McBurney MW, Rogers BJ. Isolation of male embryonal carcinoma cells and their chromosome replication patterns. Dev. Biol. 89: 503-508, 1982. PubMed: 7056443 |
| Images： |  |
| Derivation： | The P19 line was derived from an embryonal carcinoma induced in a C3H/He mouse. |
| Clinical Data： | male |
| Comments： | The line can be cloned at high efficiency in medium containing 0.1 mM 2-mercaptoethanol. The cells are pluripotent. The cell can be induced to differentiate into neural and glial like cells in the presence of 500 nM retinoic acid. In the presence of 0.5% to 1.0% dimethylsulfoxide (DMSO) the cells differentiate to form cardiac and skeletal muscle-like elements, but do not form neural or glial like cells. In the presence of both DMSO and retinoic acid, the cells differentiate as in the presence of retinoic acid alone. |
| Complete Growth Medium： | The base medium for this cell line is Alpha Minimum Essential Medium with ribonucleosides and deoxyribonucleosides. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 7.5%; fetal bovine serum to a final concentration of 2.5%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Do not allow the cells to become confluent.
Subcultivation Ratio: A subcultivation ratio of 1:10 every 2 to 3 days is recommended
Medium Renewal: Add fresh medium at least every 48 hours |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | MW McBurney |
| Deposited As： | Mus musculus |
| References： | Bennicelli JL, et al. Mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA 93: 5455-5459, 1996. PubMed: 8643596 Jones-Villeneuve EM, et al. Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells. J. Cell Biol. 94: 253-262, 1982. PubMed: 7107698 McBurney MW, Rogers BJ. Isolation of male embryonal carcinoma cells and their chromosome replication patterns. Dev. Biol. 89: 503-508, 1982. PubMed: 7056443 McBurney MW, et al. Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature 299: 165-167, 1982. PubMed: 7110336 McBurney MW, Rogers BJ. Isolation of male embryonal carcinoma cells and their chromosome replication patterns. Dev. Biol. 89: 503-508, 1982. PubMed: 7056443 |
| Cross References： | Nucleotide (GenBank) : U60288 Mus musculus proteasome subunit iota mRNA, complete cds. Nucleotide (GenBank) : NM_011968 Mus musculus proteasome (prosome, macropain) subunit, alpha type 6 (Psma6), mRNA. |