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| 产品名称： | P116.cl39 P116.c39 |
|---|---|
| 商品货号： | TS211165 |
| Organism： | Homo sapiens, human |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 2 xa0Cells contain CMV and SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | acute T cell leukemia |
| Gender： | male |
| Applications： | The P116.cl39 cell line was derived from the ZAP-70 deficient P116 cell line (ATCC CRL-2676) by transfection with a wild-type ZAP-70 expression vector, pcDNA3-mZAP. P116.cl39 cells stably expresses the ZAP-70 kinase. The cell line can be used as a control for the ZAP-70 negative P116 cells. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The P116.cl39 cell line was derived from the ZAP-70 deficient P116 cell line (ATCC CRL-2676) by transfection with a wild-type ZAP-70 expression vector, pcDNA3-mZAP. |
| Clinical Data： | male |
| Comments： | The P116.cl39 cell line was derived from the ZAP-70 deficient P116 cell line (ATCC CRL-2676) by transfection with a wild-type ZAP-70 expression vector, pcDNA3-mZAP. The vector contains cytomegalovirus (CMV) and SV40 viral DNA sequences and the neomycin resistance gene. P116.cl39 cells stably expresses the ZAP-70 kinase. The cell line can be used as a control for the ZAP-70 negative P116 cells. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.4 mg/ml G418, 90%; fetal bovine serum, 10%
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| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 2 to 3 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | RT Abraham |
| Deposited As： | human |
| References： | Williams BL, et al. Genetic evidence for differential coupling of Syk family kinases to the T-cell receptor: reconstitution studies in a ZAP-70-deficient Jurkat T- cell line. Mol. Cell. Biol. 18: 1388-1399, 1998. PubMed: 9488454 |