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微信小章| 产品名称： | Os Te |
|---|---|
| 商品货号： | TS211168 |
| Organism： | Homo sapiens, human |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | fetus |
| Gender： | female |
| Clinical Data： | The tissue donor suffered from osteogenesis imperfecta congenita (severe, broad bone type). female |
| Comments： | The tissue donor suffered from osteogenesis imperfecta congenita (severe, broad bone type). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
| Cross References： | Nucleotide (GenBank) : U50330 Human procollagen C-proteinase (pCP-2) mRNA, complete cds. Nucleotide (GenBank) : AH003197 Human (CRL 1262 variant allele) pro-alpha-1 type-I collagen gene. Nucleotide (GenBank) : K03179 Human (CRL 1262 normal allele) alpha-1 type-I collagen gene, exon 26. Nucleotide (GenBank) : K03176 Human (CRL 1262 variant allele) pro-alpha-1 type-I collagen gene, exons 31-30. Nucleotide (GenBank) : K03178 Human (CRL 1262; normal allele) pro-alpha-1 type-I collagen gene, exons 31-27. Nucleotide (GenBank) : K03177 Human (CRL 1262 variant allele) pro-alpha-1 type-1 collagen gene, exon 26, partial. |