微信小陈
微信小章| 产品名称： | OML, clone 13C |
|---|---|
| 商品货号： | TS211169 |
| Organism： | Aotus trivirgatus, monkey, Owl |
| Cell Type： | B lymphoblast; Epstein-Barr virus (EBV) transformed |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, the cells grow in dense clumps |
| Biosafety Level： | 2 Cells contain Herpesvirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | female |
| Applications： | OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The cells exhibit a karyotype consistent with the species Aotus trivirgatus; the chromosome count was 54 with an XX sex chromosome constitution., Analysis revealed a G-banding pattern of the type designated karyotype I. |
| Derivation： | OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612). |
| Clinical Data： | OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612). female |
| Antigen Expression： | CD20 + |
| Genes Expressed： | CD20 + |
| Comments： | OML, clone 13C is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from an adult female owl monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612). The resulting cells were cloned by limiting dilution. CITES Permit |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of medium. When replacing media, centrifuge cells and resuspend cell pellet in fresh medium at 5xa0x 105 viable cells/mL. Maintain cultures at cell concentrations between 5 x 105 and 2 x 106 viable cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Population Doubling Time： | 24 hrs |
| Name of Depositor： | JG Scammell |
| Year of Origin： | 1995 |
| References： | Scammell JG, et al. An EBV-transformed owl monkey B-lymphocyte cell line. In Vitro Cell. Dev. Biol. Anim. 33: 88-91, 1997. PubMed: 9081215 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |