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购买须知
| 产品名称： | OM 3-1.1 |
|---|---|
| 商品货号： | TS211172 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Animals were immunized with purified human alpha-fetoprotein. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human alpha fetoprotein (AFP, alpha-fetoprotein) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human alpha fetoprotein (AFP, alpha-fetoprotein) |
| Comments： | Animals were immunized with purified human alpha-fetoprotein. Spleen cells were fused with NS-1 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 0.005 mM 2-mercaptoethanol and HAT, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | JW Pickering |
| Deposited As： | mouse (B cell); mouse (myeloma) |