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微信小章| 产品名称： | P1.17 |
|---|---|
| 商品货号： | TS211175 |
| Organism： | Mus musculus, mouse |
| Tissue： | plasmacytoma; myeloma |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | The P1.17 line was established in tissue culture from the ADJPC5 mineral oil induced BALB/c plasmacytoma. Tested and found negative for ectromelia virus (mousepox). |
| Karyotype： | near tetraploid; modal number = 78 |
| Derivation： | The P1.17 line was established in tissue culture from the ADJPC5 mineral oil induced BALB/c plasmacytoma. |
| Antigen Expression： | H-2d; PC.1 |
| Genes Expressed： | immunoglobulin; monoclonal antibody,H-2d; PC.1 |
| Cellular Products： | immunoglobulin; monoclonal antibody |
| Comments： | The P1.17 line was established in tissue culture from the ADJPC5 mineral oil induced BALB/c plasmacytoma. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; horse serum, 10%
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | K Horibata, AW Harris |
| Deposited As： | Mus musculus |
| References： | Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516 Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914 Schubert D, et al. Immunoglobulin biosynthesis. I. A myeloma variant secreting light chain only. J. Mol. Biol. 38: 253-262, 1968. PubMed: 4180611 Schubert D, Horibata K. Immunoglobulin biosynthesis. II. Four independently isolated myeloma variants. J. Mol. Biol. 38: 263-271, 1968. PubMed: 4180612 Schubert D, Cohn M. Immunoglobulin biosynthesis. 3. Blocks in defective synthesis. J. Mol. Biol. 38: 273-288, 1968. PubMed: 4180613 Cotton RG, Milstein C. Letter: Fusion of two immunoglobulin-producing myeloma cells. Nature 244: 42-43, 1973. PubMed: 4582485 |