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微信小章| 产品名称： | Pl 1 Ut (NBL-9) |
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| 商品货号： | TS211176 |
| Organism： | Procyon lotor, raccoon |
| Tissue： | uterus |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | female |
| Applications： | This cell line was developed for studies of feline and canine viruses since the raccoon is one of the few known animals which is susceptible to both of these groups of viruses. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This cell line was developed for studies of feline and canine viruses since the raccoon is one of the few known animals which is susceptible to both of these groups of viruses. |
| Clinical Data： | female |
| Virus Resistance： | poliovirus 2; coxsackievirus A9, B5; vaccinia; adenovirus 2, 5 |
| Comments： | This cell line was developed for studies of feline and canine viruses since the raccoon is one of the few known animals which is susceptible to both of these groups of viruses. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley - Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% Carbon dioxide (CO2) |
| Name of Depositor： | AJ Kniazeff, WA Nelson-Rees, NB Darby, R Owens |
| Deposited As： | Procyon lotor |
| References： | Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |