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| 产品名称： | OKT 5 |
|---|---|
| 商品货号： | TS211178 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | In order to detect antibody production from this line by immunodiffusion a ten fold concentration of the supernatant was required. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with P3X63Ag8.U1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human T cell (human cytotoxic/suppressor T lymphocyte) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human T cell (human cytotoxic/suppressor T lymphocyte) |
| Comments： | Animals were immunized with human peripheral blood T cell. Spleen cells were fused with P3X63Ag8.U1 myeloma cells. In order to detect antibody production from this line by immunodiffusion a ten fold concentration of the supernatant was required. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 80%; fetal bovine serum, 20%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | Ortho Pharmaceutical Corporation |
| U.S. Patent Number： | |
| References： | Hoffman RA, et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 6968909 Kung PC, Goldstein G. Monoclonal antibody to human cytotoxic and suppressor T cells and methods of preparing same. US Patent 4,364,932 dated Dec 21 1982 |