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| 产品名称： | NZP-12 |
|---|---|
| 商品货号： | TS211188 |
| Organism： | Crossarchus obscurus, Cusimanse |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The cells can be propagated for at least 13 population doublings beyond frozen reference seed stock aspirate and dispense into new flasks. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 45%; Hams F12 medium, 45%; fetal bovine serum, 10%; plus the following additives: 10 ng/ml epidermal growth factor; 0.005 mg/ml insulin; 5 ng/ml selenium; 0.005 mg/ml transferrin
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature, or incubate at 37C, until the cells detach (about 5 to 10 minutes). Add fresh medium. |
| Name of Depositor： | L Munson |
| Deposited As： | Crossarchus obscurus |