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购买须知
| 产品名称： | NZP-60 |
|---|---|
| 商品货号： | TS211191 |
| Organism： | Mico melanura, black tailed marmoset |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Comments： | Mico melanura or Mico melanurus (formerly named Callithrix argentata)
The cells can be propagated for at least 12 population doublings beyond frozen reference seed stock. |
| Complete Growth Medium： | These cells are grown in Dulbeccox92s Modified Eaglex92s Medium/Hamx92s Nutrient Mixture F-12, 1:1 with 2.5 mM L-glutamine, 1.2 g/L sodium bicarbonate, 15mM HEPES and 0.5mM sodium pyruvate. Supplemented with:
This medium is formulated for use with a 5% CO2 in air atmosphere. ATCC modified and tested medium formulation (without the additional supplements or serum described above) is available as ATCC 30-2006.xa0 ATCC tested fetal bovine serum is available as ATCC 30-2020 (500ml) and ATCC 30-2021 (100ml). |
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature, or incubate at 37C, until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor： | L Munson |
| Deposited As： | Callithrix argentata |