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微信小章| 产品名称： | OK |
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| 商品货号： | TS211197 |
| Organism： | Didelphis marsupialis virginiana, opossum |
| Tissue： | kidney, cortex/proximal tubule |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Gender： | female |
| Applications： | This cell line is a suitable transfection host.
This line was originally developed for use as a source of X chromosomes in a study of X inactivation, and was subsequently found to be an excellent cell culture model for the kidney proximal tubule epithelium.
The cells display a variety of receptors in culture, and have been widely used as a model for studying those receptors.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Receptor Expression： | alpha 2 adrenergic, expressed atrial natriuretic peptide (ANP), expressed serotonin, expressed parathyroid hormone (PTH), expressed alpha 2 adrenergic; serotonin; parathyroid hormone (PTH); atrial natriuretic peptide (ANP) |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | MM Miller |
| Deposited As： | Didelphis marsupialis virginiana |
| References： | Yagil C, et al. Insulin binding, internalization, and degradation by a cultured kidney cell line. Am. J. Physiol. 254: E601-E608, 1988. PubMed: 2834959 Cole JA, et al. Regulation of sodium-dependent phosphate transport by parathyroid hormone in opossum kidney cells: adenosine 3, 5-monophosphate-dependent and -independent mechanisms. Endocrinology 122: 2981-2989, 1988. PubMed: 2836179 Nakai M, et al. Atrial natriuretic factor inhibits phosphate uptake in opossum kidney cells as a model of renal proximal tubules. Biochem. Biophys. Res. Commun. 152: 1416-1420, 1988. PubMed: 2837187 Cheng L, et al. Alpha-2-adrenergic modulation of the parathyroid hormone-inhibition of phosphate uptake in cultured renal (OK) cells. Biochem. Biophys. Res. Commun. 155: 74-82, 1988. PubMed: 2843189 Koyama H, et al. Establishment and characterization of a cell line from the American opossum (Didelphys virginiana). In Vitro 14: 239-246, 1978. PubMed: 566717 . . J. Pharmacol. 244: 571-578, 1988. |
| Cross References： | Nucleotide (GenBank) : X95475 D.virginiana mRNA for rBAT protein. |