微信小陈
微信小章| 产品名称： | NL20 |
|---|---|
| 商品货号： | TS211209 |
| Organism： | Homo sapiens, human |
| Tissue： | lung/bronchus |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain SV40 viral sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Accident victim |
| Age： | 20 years |
| Gender： | female |
| Ethnicity： | Caucasian, White |
| Applications： | The non-tumorigenic NL20 cell line and the tumorigenic NL20-TA cell line form a pair of immortal cell lines that can be used to study tumor progression. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | near diploid |
| Images： |  |
| Derivation： | NL20 (TS211209) is an immortalized, nontumorigenic human bronchial epithelial cell line derived from normal bronchus taken from an accident victim at autopsy.
The cell line was established by transfection with the origin of replication-defective SV40 large T plasmid, p129.
After three months the tumor was removed and placed in culture. At passage 3, these cells were re-injected into nude mice.
NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line. |
| Clinical Data： | 20 years Caucasian, White female |
| Tumorigenic： | No |
| Effects： | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | NL20 cells at passage 183 were inoculated into nude mice and a small slowly growing subcutaneous tumor developed from a minor clone in this otherwise stable cell line. After three months the tumor was removed and placed in culture. At passage 3, these cells were re-injected into nude mice. One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. This cell line (ATCC CRL-2504) remains tumorigenic up to at least passage 250. |
| Complete Growth Medium： | Hams F12 medium with 1.5 g/L sodium bicarbonate, 2.7 g/L glucose, 2.0 mM L-glutamine, 0.1 mM nonessential amino acids, 0.005 mg/ml insulin, 10 ng/ml epidermal growth factor, 0.001 mg/ml transferrin, 500 ng/ml hydrocortisone and 4% fetal bovine serum
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:12 to 1:20 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005.
|
| Cryopreservation： | Culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JH Schiller |
| Deposited As： | human |
| Passage History： | One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. This cell line (ATCC CRL-2504) remains tumorigenic up to at least passage 250. |
| References： | Schiller JH, et al. Phenotypic, molecular and genetic characterization of transformed human bronchial epithelial cell strains. Int. J. Oncol. 4: 461-470, 1994. Schiller JH, Bittner G. Loss of the tumorigenic phenotype with in vitro, but not in vivo, passaging of a novel series of human bronchial epithelial cell lines: possible role of an alpha 5/beta 1-integrin-fibronectin interaction. Cancer Res. : 6215-6221, 1995. PubMed: 8521416 Wittenkeller JL, et al. Comparison of spontaneous and induced mutation rates in an immortalized human bronchial epithelial cell line and its tumorigenic derivative. Oncology 54: 335-341, 1997. PubMed: 9216860 Schiller JH, et al. Karyotypic changes associated with spontaneous acquisition and loss of tumorigenicity in a human transformed bronchial epithelial cell line: evidence for in vivo selection of transformed clones. In Vitro Cell. Dev. Biol. Anim. 34: 283-289, 1998. PubMed: 9590501 |