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微信小章| 产品名称： | NMuMG |
|---|---|
| 商品货号： | TS211213 |
| Organism： | Mus musculus, mouse |
| Tissue： | mammary gland |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Strain： | NAMRU |
| Applications： | This cell line is a suitable transfection host |
| Clinical Data： | female |
| Tumorigenic： | Yes |
| Effects： | Yes, benign cystadenomas are produced in isogenic mice |
| Comments： | The cells do not express detectable retroviruses. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose and 10 mcg/ml insulin, 90%; fetal bovine serum, 10%
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| Subculturing： | Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Name of Depositor： | RB Owens |
| Deposited As： | Mus musculus |
| References： | Owens RB. Glandular epithelial cells from mice: a method for selective cultivation. J. Natl. Cancer Inst. 52: 1375-1378, 1974. PubMed: 4363715 Owens RB, et al. Epithelial cell cultures from normal glandular tissue of mice. J. Natl. Cancer Inst. 53: 261-269, 1974. PubMed: 4366196 Yingling JM, et al. Mammalian dwarfins are phosphorylated in response to transforming growth factor beta and are implicated in control of cell growth. Proc. Natl. Acad. Sci. USA 93: 8940-8944, 1996. PubMed: 8799132 |