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微信小章| 产品名称： | NR8383 AgC11x3A, NR8383.1 |
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| 商品货号： | TS211216 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | lung |
| Cell Type： | macrophage (alveolar) |
| Product Format： | frozen |
| Morphology： | macrophage |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Strain： | Sprague-Dawley |
| Applications： | The NR8383 cell line provides a homogenous source of highly responsive alveolar macarophages which can be used in vitro to study macrophage related activities. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | NR8383 (normal rat, August 3, 1983) was established from normal rat alveolar macrophage cells obtained by lung lavage. NR8383 cells were cloned and subcloned from single cells by limiting dilution, and then subcloned from soft agar three times. |
| Receptor Expression： | Fc |
| Genes Expressed： | transforming growth factor beta (TGF beta); interleukin 1 (IL-1); interleukin 6 (IL-6) |
| Cellular Products： | transforming growth factor beta (TGF beta); interleukin 1 (IL-1); interleukin 6 (IL-6) |
| Comments： | The cells were cultured in the presence of gerbil lung cell conditioned medium for approximately 8 to 9 months.
Subsequently the requirement for exogenous growth factors was lost.
The cells exhibit characteristics of macrophage cells.
Phagocytosis of zymosan and Pseudomonas aeruginosa, nonspecific esterase activity, Fc receptors, oxidative burst,IL-1, TNF beta and IL-6 secretion, and replicative response to exogenous growth factors.
The cells respond to appropriate microbial, particulate or soluble stimuli with phagocytosis and killing.
NR8383 cells respond to bleomycin by secreting latent transforming growth factor (TGF beta).
Stimulation with bleomycin also increases TGF beta mRNA expression.
These cells are sensitive to endotoxin.
LPS levels of 1 to 10 ng/mL inhibit replication by 50%.
LPS inhibition is nontoxic and reversible even after levels up to 0.001 mg/mL for extended periods. |
| Complete Growth Medium： | Hams F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; heat inactivated fetal bovine serum, 15%
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| Subculturing： | Cultures can be maintained by transferring floating cells to additional flasks. Adherent cells may be harvested by scraping. Upon reseeding, about one half of the cells will re-attach. Cultures are most successful when set up at a floating cell concentration of 1- 4 x 105 viable cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | RJ Helmke |
| Deposited As： | Rattus sp. |
| Year of Origin： | August 3, 1983 |
| References： | Hidalgo HA, et al. Pneumocystis carinii induces an oxidative burst in alveolar macrophages. Infect. Immun. 60: 1-7, 1992. PubMed: 1729174 Helmke RJ, et al. A continuous alveolar macrophage cell line: comparisons with freshly derived alveolar macrophages. In Vitro Cell. Dev. Biol. 25: 44-48, 1989. PubMed: 2914814 Helmke RJ, et al. From growth factor dependence to growth factor responsiveness: the genesis of an alveolar macrophage cell line. In Vitro Cell. Dev. Biol. 23: 567-574, 1987. PubMed: 3497918 Limper AH, Standing JE. Vitronectin interacts with Candida albicans and augments organism attachment to the NR8383 macrophage cell line. Immunol. Lett. 42: 139-144, 1994. PubMed: 7534269 Hidalgo HA, et al. The effects of cyclosporine and dexamethasone on an alveolar macrophage cell line (NR8383). Transplantation 53: 620-623, 1992. PubMed: 1549855 Denholm EM, Rollins SM. Expression and secretion of transforming growth factor-beta by bleomycin-stimulated rat alveolar macrophages. Am. J. Physiol. 264: L36-L42, 1993. PubMed: 7679254 Krieg DP, et al. Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect. Immun. 56: 3173-3169, 1988. PubMed: 3141284 Sherman MP, et al. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem. Biophys. Res. Commun. 191: 1301-1308, 1993. PubMed: 7682068 Griscavage JM, et al. Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. J. Immunol. 151: 6329-6337, 1993. PubMed: 7504017 Henderson SA, et al. Nitric oxide reduces early growth response-1 gene expression in rat lung macrophages treated with interferon-gamma and lipopolysaccharide. J. Biol. Chem. 269: 25239-25242, 1994. PubMed: 7523382 Huang S, et al. Rat KC cDNA cloning and mRNA expression in lung macrophages and fibroblasts. Biochem. Biophys. Res. Commun. 184: 922-929, 1992. PubMed: 1374243 |