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微信小章| 产品名称： | NFPE |
|---|---|
| 商品货号： | TS211220 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | embryonal carcinoma |
| Age： | embryo; blastocyst |
| Strain： | BALB/c |
| Applications： | The line was established from NF-1 mouse embryonal carcinoma established by Fox et al. Phenotypically, the cells resemble primitive endoderm from 5 to 6 day mouse embryos. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The line was established from NF-1 mouse embryonal carcinoma established by Fox et al. (see ATCC CRL-2075). |
| Comments： | The line was established from NF-1 mouse embryonal carcinoma established by Fox et al. (see ATCC CRL-2075). Phenotypically, the cells resemble primitive endoderm from 5 to 6 day mouse embryos. They are reactive with the SSEA-3 monoclonal antibody. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:8 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | I Damjanov |
| Deposited As： | Mus musculus |
| References： | Fox N, et al. Male murine embryonal carcinoma cell line selectively metastatic to the ovaries and adrenals. Virchows Arch. B Cell Pathol. 43: 241-251, 1983. PubMed: 6137898 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |