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| 产品名称： | NFS-5 C-1 |
|---|---|
| 商品货号： | TS211221 |
| Organism： | Mus musculus, mouse |
| Cell Type： | pre-B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | NFS/N |
| Applications： | The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus. They are negative for ThB, surface immunoglobulin (sIg) and Ia. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus. |
| Antigen Expression： | CD5 +; CD32 +; CD45R + |
| Genes Expressed： | ecotropic retrovirus; mink cell focus forming retrovirus,CD5 +; CD32 +; CD45R + |
| Cellular Products： | ecotropic retrovirus; mink cell focus forming retrovirus |
| Comments： | The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus. The cells bear a pattern of markers consistent with identification as large pre-B lymphoblasts (Ly 17, CD32. Ly 1, CD5. Lyb 2, Ly 5(B220), CD45R). They are negative for ThB, surface immunoglobulin (sIg) and Ia. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Modified Dulbeccos medium with 0.002 mg/ml transferrin and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | WJ Davidson, HC Morse |
| Deposited As： | Mus musculus |
| References： | Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201 |