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微信小章| 产品名称： | Neuro-2a |
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| 商品货号： | TS211223 |
| Organism： | Mus musculus, mouse |
| Tissue： | brain |
| Cell Type： | neuroblast |
| Product Format： | frozen |
| Morphology： | neuronal and amoeboid stem cells |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | neuroblastoma |
| Strain： | A |
| Applications： | This cell line is a suitable transfection host. The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubular protein . The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies.xa0 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 95; range = 59 to 193. Karyotype unstable within a stemline range of 94 to 98 chromosomes. All the cells contain 6 to 10 large chromosomes with median or submedian centromeres and 2 to 4 minute chromosomes. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Images： |  |
| Derivation： | Clone Neuro-2a was established by R.J. Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse. This tumor line, designated C1300, was obtained from the Jackson Laboratory, Bar Harbor, Maine |
| Antigen Expression： | H-2, a haplotype; Mus musculus, expressed |
| Genes Expressed： | acetylcholinesterase, tubulin |
| Cellular Products： | acetylcholinesterase tubulin |
| Virus Susceptibility： | Herpes simplex virus
Vesicular stomatitis virus Human poliovirus 1 |
| Comments： | Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 1 to 2 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | RJ Klebe |
| Deposited As： | Mus musculus |
| References： | Olmsted JB, et al. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc. Natl. Acad. Sci. USA 65: 129-136, 1970. PubMed: 5263744 Klebe RJ, Ruddle FH. Neuroblastoma: Cell culture analysis of a differentiating stem cell system. J. Cell Biol. 43: 69A, 1969. Naslavsky N, et al. Characterization of detergent-insoluble complexes containing the cellular prion protein and its scrapie isoform. J. Biol. Chem. 272: 6324-6331, 1997. PubMed: 9045652 Kaneko K, et al. Evidence for protein X binding to a discontinuous epitope on the cellular prion protein during scrapie prion propagation. Proc. Natl. Acad. Sci. USA 94: 10069-10074, 1997. PubMed: 9294164 Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614 |
| Cross References： | Nucleotide (GenBank) : AF209436 Mus musculus c-ret proto-oncogene mRNA, complete cds. |