微信小陈
微信小章| 产品名称： | NFS-25 C-3 |
|---|---|
| 商品货号： | TS211224 |
| Organism： | Mus musculus, mouse |
| Cell Type： | pre-B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | NFS.C58v-1 |
| Applications： | The NFS-25 C-3 cell line was derived from a spontaneous lymphoma. They are negative for ThB, surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The NFS-25 C-3 cell line was derived from a spontaneous lymphoma. |
| Antigen Expression： | CD5 +; CD32 +; CD45R + |
| Genes Expressed： | CD5 +; CD32 +; CD45R + |
| Comments： | The NFS-25 C-3 cell line was derived from a spontaneous lymphoma. The cells bear markers consistent with identification as large pre-B lymphoblasts (Ly 17, CD32), (Ly 1, CD5), (Lyb 2, Ly 5(B220), CD45R). They are negative for ThB, surface immunoglobulin (sIg) and Ia, tested, and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 10 mM HEPES, 3 mM glutamine, 1 mM oxaloacetic acid, 0.2 units/ml insulin, 0.5 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol, 2 mcg/ml transferrin, 80%; NCTC medium 109, 10%; fetal bovine serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | WJ Davidson, HC Morse |
| Deposited As： | Mus musculus |
| References： | Davidson WF, et al. A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |