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微信小章| 产品名称： | NCTC 4206 |
|---|---|
| 商品货号： | TS211228 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | peritoneum |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Virus Resistance： | poliovirus 3 |
| Comments： | Grows in chemically defined medium. |
| Complete Growth Medium： | NCTC 135 medium, 100%
|
| Subculturing： | Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: 1:2 is recommended
Medium Renewal: 3 times per week |
| Cryopreservation： | Culture medium, 95%; glycerol, 5%. |
| Culture Conditions： | Temperature:xa037°C |
| Name of Depositor： | VJ Evans |
| Deposited As： | Cricetulus griseus |
| References： | Evans VJ, et al. Chemically defined media for cultivation of long-term cell strains from four mammalian species. Exp. Cell Res. 36: 439-474, 1964. PubMed: 14242231 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |