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微信小章| 产品名称： | NCTC 4093 |
|---|---|
| 商品货号： | TS211229 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Strain： | C57BL/KaLw |
| Applications： | 10% horse serum may be used instead of fetal bovine serum in medium. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Antigen Expression： | H-2d |
| Genes Expressed： | H-2d |
| Virus Resistance： | poliovirus 1 |
| Comments： | 10% horse serum may be used instead of fetal bovine serum in medium. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | NCTC 135 medium, 90%; fetal bovine serum, 10%; adjust pH to circa 7.2 with humidified 5 to 10% CO2 in air
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| Subculturing： | Subcultures can be prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Alternatively, a standard trypsinization procedure may be used.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2 |
| Name of Depositor： | KK Sanford |
| Deposited As： | Mus musculus |
| References： | Kampine JP, et al. Diagnosis of gauchers disease and niemann-pick disease with small samples of venous blood. Science 155: 86-88, 1967. PubMed: 6015567 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |